AUTHORS
Vincent Hahaut, Dinko Pavlinic, Walter Carbone, Sven Schuierer, Pierre Balmer, Mathieu Quinodoz, Magdalena Renner, Guglielmo Roma, Cameron S Cowan, Simone Picelli
Nat Biotechnol. 2022 Oct;40(10):1447-1451. doi: 10.1038/s41587-022-01312-3. Epub 2022 May 30.
ABSTRACT
We present FLASH-seq (FS), a full-length single-cell RNA sequencing (scRNA-seq) method with increased sensitivity and reduced hands-on time compared to Smart-seq3. The entire FS protocol can be performed in ~4.5 hours, is simple to automate and can be easily miniaturized to decrease resource consumption. The FS protocol can also use unique molecular identifiers (UMIs) for molecule counting while displaying reduced strand-invasion artifacts. FS will be especially useful for characterizing gene expression at high resolution across multiple samples.
PMID:35637419 | PMC:PMC9546769 | DOI:10.1038/s41587-022-01312-3
