Single cell technologies enable the analysis of thousands of single cells in just one experiment, generating an unprecedented amount of data. The field is less than ten years old and new methods and algorithms for data analysis are being published almost every week.

The single-cell genomics platform develops new single-cell based transcriptomic and multiomic methods to improve data quality, increase the throughput, and reduce costs. Additionally, the platform provides IOB’s clinicians and basic researchers with guidance, support and advice for implementing single-cell sequencing technologies for their projects.

The platform offers the latest technologies in the genomics field and has made significant contributions to understanding fundamental molecular mechanisms in healthy and diseased individuals.

Single Cell and Nuclei Dissociation

• Qiagen RNA extraction Kits
• Miltenyi Biosciences Tissue Dissociation Kits
• Salty-EZ-based protocols (Adapted from Martelotto, LG 2021)

Technologies

• 10x Genomics Single Cell Gene Expression 3’ and 5’ (with Feature Barcoding and Sample Multiplexing)
• 10x Genomics Single Cell Multiome
• FLASH-seq (plate format only)
• G&T-seq (plate format only)
• PATCH-seq
• Bulk FLASH-seq
• Curio Biosciences Seeker (Spatial Transcriptomics)

Technology Overview

Quality control

• DNA/RNA Quantification with Qubit or Nanodrop
• DNA/RNA size and quality assessment with Bioanalyzer
• Plate quantification with Hidex Plate Reader

Equipment

• 10x Chromium Controller and 10x Chromium X
• BD Rhapsody Single-Cell Analysis System
• Illumina NextSeq550
• Oxford Nanopore P2 Solo
• Oxford Nanopore Minion
• TECAN Fluent 780
• I.DOT Nanodispenser
• Qubit v4 Fluorometer
• Agilent 2100 Bioanalyzer
• MoxiGo 532
• LunaFX
• Atrandi Biosciences Onyx