AUTHORS
Fabian F Voigt, Daniel Kirschenbaum, Evgenia Platonova, Stéphane Pagès, Robert A A Campbell, Rahel Kastli, Martina Schaettin, Ladan Egolf, Alexander van der Bourg, Philipp Bethge, Karen Haenraets, Noémie Frézel, Thomas Topilko, Paola Perin, Daniel Hillier, Sven Hildebrand, Anna Schueth, Alard Roebroeck, Botond Roska, Esther T Stoeckli, Roberto Pizzala, Nicolas Renier, Hanns Ulrich Zeilhofer, Theofanis Karayannis, Urs Ziegler, Laura Batti, Anthony Holtmaat, Christian Lüscher, Adriano Aguzzi, Fritjof Helmchen
Nat Methods. 2019 Nov;16(11):1105-1108. doi: 10.1038/s41592-019-0554-0. Epub 2019 Sep 16.
ABSTRACT
Light-sheet microscopy is an ideal technique for imaging large cleared samples; however, the community is still lacking instruments capable of producing volumetric images of centimeter-sized cleared samples with near-isotropic resolution within minutes. Here, we introduce the mesoscale selective plane-illumination microscopy initiative, an open-hardware project for building and operating a light-sheet microscope that addresses these challenges and is compatible with any type of cleared or expanded sample ( www.mesospim.org ).
PMID:31527839 | PMC:PMC6824906 | DOI:10.1038/s41592-019-0554-0
